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+--------+-------------------------+-------------------------+--------+--------+
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|00000010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
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|00000680| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00000690| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000006a0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
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|000006c0| 2d 2d 2d 2d 2d 0d 2d 2d | 0d 2d 2d 20 4d 61 74 65 |-----.--|.-- Mate|
|000006d0| 72 69 61 6c 73 20 26 20 | 4d 65 74 68 6f 64 73 2c |rials & |Methods,|
|000006e0| 20 61 20 48 79 70 65 72 | 63 61 72 64 20 73 74 61 | a Hyper|card sta|
|000006f0| 63 6b 20 66 6f 72 20 74 | 68 65 20 4d 61 63 69 6e |ck for t|he Macin|
|00000700| 74 6f 73 68 20 63 6f 6d | 70 75 74 65 72 0d 2d 2d |tosh com|puter.--|
|00000710| 20 22 6d 69 6e 69 4d 26 | 4d 22 20 48 79 70 65 72 | "miniM&|M" Hyper|
|00000720| 63 61 72 64 20 69 6e 74 | 65 72 66 61 63 65 20 76 |card int|erface v|
|00000730| 65 72 73 69 6f 6e 20 2d | 20 69 6e 20 64 65 76 65 |ersion -| in deve|
|00000740| 6c 6f 70 6d 65 6e 74 0d | 2d 2d 0d 2d 2d 20 62 79 |lopment.|--.-- by|
|00000750| 20 4a 61 6d 65 73 20 57 | 2e 20 42 72 6f 77 6e 0d | James W|. Brown.|
|00000760| 2d 2d 20 42 69 6f 6c 6f | 67 79 20 44 65 70 61 72 |-- Biolo|gy Depar|
|00000770| 74 6d 65 6e 74 0d 2d 2d | 20 49 6e 64 69 61 6e 61 |tment.--| Indiana|
|00000780| 20 55 6e 69 76 65 72 73 | 69 74 79 0d 2d 2d 20 42 | Univers|ity.-- B|
|00000790| 6c 6f 6f 6d 69 6e 67 74 | 6f 6e 2c 20 49 4e 20 34 |loomingt|on, IN 4|
|000007a0| 37 34 30 35 0d 2d 2d 20 | 55 53 41 0d 2d 2d 0d 2d |7405.-- |USA.--.-|
|000007b0| 2d 20 42 69 74 4e 65 74 | 20 61 64 64 72 65 73 73 |- BitNet| address|
|000007c0| 20 3a 20 42 52 4f 57 4e | 4a 57 40 49 55 42 41 43 | : BROWN|JW@IUBAC|
|000007d0| 53 0d 2d 2d 0d 0d 6f 6e | 20 6f 70 65 6e 53 74 61 |S.--..on| openSta|
|000007e0| 63 6b 0d 67 6c 6f 62 61 | 6c 20 4d 45 4e 55 2c 54 |ck.globa|l MENU,T|
|000007f0| 48 45 4c 49 53 54 2c 63 | 75 72 72 65 6e 74 57 69 |HELIST,c|urrentWi|
|00000800| 6e 64 6f 77 0d 70 75 74 | 20 22 4f 6e 65 20 6d 6f |ndow.put| "One mo|
|00000810| 6d 65 6e 74 2c 20 70 6c | 65 61 73 65 2e 2e 2e 2e |ment, pl|ease....|
|00000820| 22 20 69 6e 74 6f 20 6d | 73 67 0d 73 65 74 20 74 |" into m|sg.set t|
|00000830| 68 65 20 63 75 72 73 6f | 72 20 74 6f 20 34 0d 2d |he curso|r to 4.-|
|00000840| 2d 20 49 6e 69 74 69 61 | 6c 69 7a 65 20 74 68 65 |- Initia|lize the|
|00000850| 20 6d 65 6e 75 0d 70 75 | 74 20 4e 65 77 4d 65 6e | menu.pu|t NewMen|
|00000860| 75 28 22 6d 69 6e 69 4d | 26 4d 22 2c 20 22 41 62 |u("miniM|&M", "Ab|
|00000870| 6f 75 74 20 6d 69 6e 69 | 4d 26 4d 2e 2e 2e 22 2c |out mini|M&M...",|
|00000880| 22 28 2d 22 2c 22 43 68 | 6f 6f 73 65 20 63 61 72 |"(-","Ch|oose car|
|00000890| 64 2e 2e 2e 22 2c 22 28 | 2d 22 2c 22 52 65 61 64 |d...","(|-","Read|
|000008a0| 20 54 65 78 74 20 66 69 | 6c 65 2e 2e 2e 22 2c 22 | Text fi|le...","|
|000008b0| 43 72 65 61 74 65 20 54 | 65 78 74 20 66 69 6c 65 |Create T|ext file|
|000008c0| 2e 2e 2e 22 2c 22 52 65 | 61 64 20 4d 26 4d 20 66 |...","Re|ad M&M f|
|000008d0| 69 6c 65 2e 2e 2e 22 2c | 22 43 72 65 61 74 65 20 |ile...",|"Create |
|000008e0| 4d 26 4d 20 66 69 6c 65 | 2e 2e 2e 22 2c 22 28 2d |M&M file|...","(-|
|000008f0| 22 2c 22 50 72 69 6e 74 | 20 70 72 6f 63 65 64 75 |","Print| procedu|
|00000900| 72 65 22 2c 22 50 72 69 | 6e 74 20 41 4c 4c 20 70 |re","Pri|nt ALL p|
|00000910| 72 6f 63 65 64 75 72 65 | 73 2e 2e 2e 22 2c 22 28 |rocedure|s...","(|
|00000920| 2d 22 2c 22 49 6e 74 72 | 6f 64 75 63 74 69 6f 6e |-","Intr|oduction|
|00000930| 22 2c 22 4d 61 74 65 72 | 69 61 6c 73 22 2c 22 4d |","Mater|ials","M|
|00000940| 65 74 68 6f 64 73 22 2c | 29 20 69 6e 74 6f 20 4d |ethods",|) into M|
|00000950| 45 4e 55 0d 45 6e 61 62 | 6c 65 4d 65 6e 75 20 4d |ENU.Enab|leMenu M|
|00000960| 45 4e 55 2c 32 2c 46 41 | 4c 53 45 0d 45 6e 61 62 |ENU,2,FA|LSE.Enab|
|00000970| 6c 65 4d 65 6e 75 20 4d | 45 4e 55 2c 34 2c 46 41 |leMenu M|ENU,4,FA|
|00000980| 4c 53 45 0d 45 6e 61 62 | 6c 65 4d 65 6e 75 20 4d |LSE.Enab|leMenu M|
|00000990| 45 4e 55 2c 39 2c 46 41 | 4c 53 45 0d 45 6e 61 62 |ENU,9,FA|LSE.Enab|
|000009a0| 6c 65 4d 65 6e 75 20 4d | 45 4e 55 2c 31 32 2c 46 |leMenu M|ENU,12,F|
|000009b0| 41 4c 53 45 0d 2d 2d 4c | 6f 61 64 20 75 70 20 54 |ALSE.--L|oad up T|
|000009c0| 48 45 4c 49 53 54 0d 69 | 66 20 74 68 65 20 6e 61 |HELIST.i|f the na|
|000009d0| 6d 65 20 6f 66 20 74 68 | 69 73 20 63 61 72 64 20 |me of th|is card |
|000009e0| 69 73 20 6e 6f 74 20 22 | 48 45 4c 50 21 22 20 74 |is not "|HELP!" t|
|000009f0| 68 65 6e 0d 70 75 73 68 | 20 63 61 72 64 0d 67 6f |hen.push| card.go|
|00000a00| 20 74 6f 20 63 61 72 64 | 20 22 68 65 6c 70 21 22 | to card| "help!"|
|00000a10| 0d 70 75 74 20 66 69 65 | 6c 64 20 22 6c 69 73 74 |.put fie|ld "list|
|00000a20| 46 69 65 6c 64 22 20 69 | 6e 74 6f 20 54 48 45 4c |Field" i|nto THEL|
|00000a30| 49 53 54 0d 70 6f 70 20 | 63 61 72 64 0d 65 6c 73 |IST.pop |card.els|
|00000a40| 65 0d 70 75 74 20 66 69 | 65 6c 64 20 22 6c 69 73 |e.put fi|eld "lis|
|00000a50| 74 46 69 65 6c 64 22 20 | 69 6e 74 6f 20 54 48 45 |tField" |into THE|
|00000a60| 4c 49 53 54 0d 65 6e 64 | 20 69 66 0d 64 6f 4d 65 |LIST.end| if.doMe|
|00000a70| 6e 75 20 22 49 6e 74 72 | 6f 64 75 63 74 69 6f 6e |nu "Intr|oduction|
|00000a80| 22 0d 70 75 74 20 22 69 | 6e 74 72 6f 64 75 63 74 |".put "i|ntroduct|
|00000a90| 69 6f 6e 22 20 69 6e 74 | 6f 20 63 75 72 72 65 6e |ion" int|o curren|
|00000aa0| 74 57 69 6e 64 6f 77 0d | 70 75 74 20 65 6d 70 74 |tWindow.|put empt|
|00000ab0| 79 20 69 6e 74 6f 20 6d | 73 67 0d 68 69 64 65 20 |y into m|sg.hide |
|00000ac0| 6d 73 67 0d 65 6e 64 20 | 6f 70 65 6e 73 74 61 63 |msg.end |openstac|
|00000ad0| 6b 0d 0d 6f 6e 20 63 6c | 6f 73 65 53 74 61 63 6b |k..on cl|oseStack|
|00000ae0| 0d 67 6c 6f 62 61 6c 20 | 4d 45 4e 55 2c 54 48 45 |.global |MENU,THE|
|00000af0| 4c 49 53 54 0d 2d 2d 20 | 43 6c 65 61 6e 20 75 70 |LIST.-- |Clean up|
|00000b00| 20 61 66 74 65 72 20 74 | 68 65 20 6d 65 6e 75 0d | after t|he menu.|
|00000b10| 70 75 74 20 44 65 6c 65 | 74 65 4d 65 6e 75 28 4d |put Dele|teMenu(M|
|00000b20| 45 4e 55 29 20 69 6e 74 | 6f 20 4d 45 4e 55 0d 2d |ENU) int|o MENU.-|
|00000b30| 2d 20 75 70 64 61 74 65 | 20 6c 69 73 74 46 69 65 |- update| listFie|
|00000b40| 6c 64 0d 67 6f 20 74 6f | 20 63 61 72 64 20 22 68 |ld.go to| card "h|
|00000b50| 65 6c 70 21 22 0d 70 75 | 74 20 54 48 45 4c 49 53 |elp!".pu|t THELIS|
|00000b60| 54 20 69 6e 74 6f 20 66 | 69 65 6c 64 20 22 6c 69 |T into f|ield "li|
|00000b70| 73 74 46 69 65 6c 64 22 | 0d 65 6e 64 20 63 6c 6f |stField"|.end clo|
|00000b80| 73 65 53 74 61 63 6b 0d | 0d 6f 6e 20 69 64 6c 65 |seStack.|.on idle|
|00000b90| 0d 2d 2d 20 49 6e 20 63 | 61 73 65 20 74 68 65 20 |.-- In c|ase the |
|00000ba0| 6d 65 6e 75 20 69 73 20 | 68 69 64 64 65 6e 20 66 |menu is |hidden f|
|00000bb0| 6f 72 20 73 6f 6d 65 20 | 72 65 61 73 6f 6e 0d 67 |or some |reason.g|
|00000bc0| 6c 6f 62 61 6c 20 4d 45 | 4e 55 2c 4c 41 53 54 54 |lobal ME|NU,LASTT|
|00000bd0| 49 43 4b 0d 69 66 20 28 | 74 68 65 20 74 69 63 6b |ICK.if (|the tick|
|00000be0| 73 20 2d 20 4c 41 53 54 | 54 49 43 4b 29 3e 31 32 |s - LAST|TICK)>12|
|00000bf0| 30 20 74 68 65 6e 0d 70 | 75 74 20 74 68 65 20 74 |0 then.p|ut the t|
|00000c00| 69 63 6b 73 20 69 6e 74 | 6f 20 4c 41 53 54 54 49 |icks int|o LASTTI|
|00000c10| 43 4b 0d 53 68 6f 77 4d | 65 6e 75 28 4d 45 4e 55 |CK.ShowM|enu(MENU|
|00000c20| 29 0d 65 6e 64 20 69 66 | 0d 70 61 73 73 20 69 64 |).end if|.pass id|
|00000c30| 6c 65 0d 65 6e 64 20 69 | 64 6c 65 0d 0d 6f 6e 20 |le.end i|dle..on |
|00000c40| 6f 70 65 6e 43 61 72 64 | 0d 2d 2d 20 44 6f 6e 27 |openCard|.-- Don'|
|00000c50| 74 20 6c 65 61 76 65 20 | 74 68 65 20 77 69 6e 64 |t leave |the wind|
|00000c60| 6f 77 73 20 73 63 72 6f | 6c 6c 65 64 20 74 6f 20 |ows scro|lled to |
|00000c70| 74 68 65 20 62 6f 74 74 | 6f 6d 21 0d 73 65 74 20 |the bott|om!.set |
|00000c80| 74 68 65 20 73 63 72 6f | 6c 6c 20 6f 66 20 66 69 |the scro|ll of fi|
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|00000cd0| 64 20 35 20 74 6f 20 30 | 0d 73 65 74 20 74 68 65 |d 5 to 0|.set the|
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|00000cf0| 20 36 20 74 6f 20 30 0d | 65 6e 64 20 6f 70 65 6e | 6 to 0.|end open|
|00000d00| 43 61 72 64 0d 0d 6f 6e | 20 64 6f 4d 65 6e 75 20 |Card..on| doMenu |
|00000d10| 57 48 49 43 48 0d 2d 2d | 20 70 69 63 6b 20 6f 75 |WHICH.--| pick ou|
|00000d20| 74 20 74 68 65 20 6d 65 | 6e 75 20 63 68 6f 69 63 |t the me|nu choic|
|00000d30| 65 20 26 20 64 69 72 65 | 63 74 20 74 68 65 20 61 |e & dire|ct the a|
|00000d40| 63 74 69 6f 6e 0d 67 6c | 6f 62 61 6c 20 50 41 53 |ction.gl|obal PAS|
|00000d50| 53 4f 4e 0d 70 75 74 20 | 66 61 6c 73 65 20 69 6e |SON.put |false in|
|00000d60| 74 6f 20 50 41 53 53 4f | 4e 0d 69 66 20 57 48 49 |to PASSO|N.if WHI|
|00000d70| 43 48 20 69 73 20 22 41 | 62 6f 75 74 20 6d 69 6e |CH is "A|bout min|
|00000d80| 69 4d 26 4d 2e 2e 2e 22 | 20 74 68 65 6e 20 61 62 |iM&M..."| then ab|
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|00000da0| 48 20 69 73 20 22 43 68 | 6f 6f 73 65 20 63 61 72 |H is "Ch|oose car|
|00000db0| 64 2e 2e 2e 22 20 74 68 | 65 6e 20 63 68 6f 6f 73 |d..." th|en choos|
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|00000dd0| 49 43 48 20 69 73 20 22 | 52 65 61 64 20 74 65 78 |ICH is "|Read tex|
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|00000df0| 69 6d 70 6f 72 74 54 65 | 78 74 0d 65 6c 73 65 20 |importTe|xt.else |
|00000e00| 69 66 20 57 48 49 43 48 | 20 69 73 20 22 43 72 65 |if WHICH| is "Cre|
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|00000e20| 22 20 74 68 65 6e 20 63 | 72 65 61 74 65 54 65 78 |" then c|reateTex|
|00000e30| 74 0d 65 6c 73 65 20 69 | 66 20 57 48 49 43 48 20 |t.else i|f WHICH |
|00000e40| 69 73 20 22 52 65 61 64 | 20 4d 26 4d 20 66 69 6c |is "Read| M&M fil|
|00000e50| 65 2e 2e 2e 22 20 74 68 | 65 6e 20 69 6d 70 6f 72 |e..." th|en impor|
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|00000e70| 69 66 20 57 48 49 43 48 | 20 69 73 20 22 50 72 69 |if WHICH| is "Pri|
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|00002a00| 74 20 30 20 69 6e 74 6f | 20 49 4e 44 45 58 0d 72 |t 0 into| INDEX.r|
|00002a10| 65 70 65 61 74 20 66 6f | 72 20 74 68 65 20 6e 75 |epeat fo|r the nu|
|00002a20| 6d 62 65 72 20 6f 66 20 | 63 61 72 64 73 20 69 6e |mber of |cards in|
|00002a30| 20 74 68 69 73 20 62 61 | 63 6b 67 72 6f 75 6e 64 | this ba|ckground|
|00002a40| 0d 61 64 64 20 31 20 74 | 6f 20 49 4e 44 45 58 0d |.add 1 t|o INDEX.|
|00002a50| 67 6f 20 74 6f 20 63 61 | 72 64 20 49 4e 44 45 58 |go to ca|rd INDEX|
|00002a60| 20 6f 66 20 74 68 69 73 | 20 62 61 63 6b 67 72 6f | of this| backgro|
|00002a70| 75 6e 64 0d 63 72 65 61 | 74 65 4d 61 6e 64 4d 66 |und.crea|teMandMf|
|00002a80| 69 6c 65 0d 65 6e 64 20 | 72 65 70 65 61 74 0d 65 |ile.end |repeat.e|
|00002a90| 6e 64 20 64 75 6d 70 46 | 69 6c 65 73 0d 0d 0d 0d |nd dumpF|iles....|
|00002aa0| 00 20 74 6f 20 63 61 72 | 64 20 49 4e 44 45 58 20 |. to car|d INDEX |
|00002ab0| 6f 66 20 74 68 69 73 20 | 62 61 63 6b 67 72 6f 75 |of this |backgrou|
|00002ac0| 6e 64 0d 63 72 65 61 74 | 65 4d 61 6e 64 4d 66 69 |nd.creat|eMandMfi|
|00002ad0| 6c 65 0d 65 6e 64 20 72 | 65 70 65 61 74 0d 65 6e |le.end r|epeat.en|
|00002ae0| 64 20 64 75 6d 70 46 69 | 6c 65 73 0d 0d 0d 0d 00 |d dumpFi|les.....|
|00002af0| 69 65 6c 64 20 63 6f 6d | 6d 65 6e 74 73 0d 65 6e |ield com|ments.en|
|00002b00| 64 20 73 68 6f 77 49 6e | 74 72 6f 0d 0d 6f 6e 20 |d showIn|tro..on |
|00002b10| 73 68 6f 77 4d 61 74 65 | 72 69 61 6c 73 0d 7a 61 |showMate|rials.za|
|00002b20| 70 57 69 6e 64 6f 77 73 | 0d 73 68 6f 77 20 62 61 |pWindows|.show ba|
|00002b30| 63 6b 67 72 6f 75 6e 64 | 20 66 69 65 6c 64 20 72 |ckground| field r|
|00002b40| 65 63 69 70 65 73 0d 65 | 6e 64 20 73 68 6f 77 4d |ecipes.e|nd showM|
|00002b50| 61 74 65 72 69 61 6c 73 | 0d 0d 6f 6e 20 73 68 6f |aterials|..on sho|
|00002b60| 77 4d 65 74 68 6f 64 73 | 0d 7a 61 70 57 69 6e 64 |wMethods|.zapWind|
|00002b70| 6f 77 73 0d 73 68 6f 77 | 20 62 61 63 6b 67 72 6f |ows.show| backgro|
|00002b80| 75 6e 64 20 66 69 65 6c | 64 20 50 72 6f 63 65 64 |und fiel|d Proced|
|00002b90| 75 72 65 0d 65 6e 64 20 | 73 68 6f 77 4d 65 74 68 |ure.end |showMeth|
|00002ba0| 6f 64 73 0d 0d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |ods..---|--------|
|00002bb0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002bc0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002bd0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002be0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 0d 0d 00 68 69 63 |--------|--...hic|
|00002bf0| 0d 0d 6f 6e 20 73 68 6f | 77 49 6e 74 72 6f 0d 7a |..on sho|wIntro.z|
|00002c00| 61 70 57 69 6e 64 6f 77 | 73 0d 73 68 6f 77 20 62 |apWindow|s.show b|
|00002c10| 61 63 6b 67 72 6f 75 6e | 64 20 66 69 65 6c 64 20 |ackgroun|d field |
|00002c20| 63 6f 6d 6d 65 6e 74 73 | 0d 65 6e 64 20 73 68 6f |comments|.end sho|
|00002c30| 77 49 6e 74 72 6f 0d 0d | 6f 6e 20 73 68 6f 77 4d |wIntro..|on showM|
|00002c40| 61 74 65 72 69 61 6c 73 | 0d 7a 61 70 57 69 6e 64 |aterials|.zapWind|
|00002c50| 6f 77 73 0d 73 68 6f 77 | 20 62 61 63 6b 67 72 6f |ows.show| backgro|
|00002c60| 75 6e 64 20 66 69 65 6c | 64 20 72 65 63 69 70 65 |und fiel|d recipe|
|00002c70| 73 0d 65 6e 64 20 73 68 | 6f 77 4d 61 74 65 72 69 |s.end sh|owMateri|
|00002c80| 61 6c 73 0d 0d 6f 6e 20 | 73 68 6f 77 4d 65 74 68 |als..on |showMeth|
|00002c90| 6f 64 73 0d 7a 61 70 57 | 69 6e 64 6f 77 73 0d 73 |ods.zapW|indows.s|
|00002ca0| 68 6f 77 20 62 61 63 6b | 67 72 6f 75 6e 64 20 66 |how back|ground f|
|00002cb0| 69 65 6c 64 20 50 72 6f | 63 65 64 75 72 65 0d 65 |ield Pro|cedure.e|
|00002cc0| 6e 64 20 73 68 6f 77 4d | 65 74 68 6f 64 73 0d 0d |nd showM|ethods..|
|00002cd0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002ce0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002cf0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002d00| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00002d10| 2d 2d 2d 2d 2d 0d 0d 00 | 00 00 00 00 00 00 00 00 |-----...|........|
|00002d20| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d40| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d50| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d80| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002d90| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002da0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002db0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002dc0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002dd0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002de0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002df0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e00| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e10| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e20| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e30| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e40| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e50| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e60| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e70| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002e80| 00 00 02 00 4d 41 53 54 | ff ff ff ff 00 00 00 00 |....MAST|........|
|00002e90| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002ea0| 00 02 a8 40 00 03 c4 22 | 00 02 04 a6 00 02 2e 76 |...@..."|.......v|
|00002eb0| 00 02 2a b2 00 1b ce 2d | 00 01 80 8a 00 04 08 65 |..*....-|.......e|
|00002ec0| 00 04 2d c1 00 00 00 00 | 00 04 b6 d7 00 00 00 00 |..-.....|........|
|00002ed0| 00 04 fe 0d 00 05 ad 2b | 00 06 2d 09 00 06 6b d0 |.......+|..-...k.|
|00002ee0| 00 07 09 59 00 07 e8 75 | 00 08 77 ce 00 08 f9 09 |...Y...u|..w.....|
|00002ef0| 00 09 28 b6 00 09 7e 33 | 00 1b d8 06 00 0a 7f 55 |..(...~3|.......U|
|00002f00| 00 0a e9 80 00 0b 80 05 | 00 0b f7 6e 00 0c 50 ed |........|...n..P.|
|00002f10| 00 0c ed 0a 00 0d 3e 7d | 00 0d 76 f2 00 0d b4 19 |......>}|..v.....|
|00002f20| 00 0d f9 fb 00 0e 50 82 | 00 10 0e 98 00 10 7b e6 |......P.|......{.|
|00002f30| 00 10 b6 fa 00 10 e1 ac | 00 11 3f 51 00 00 00 00 |........|..?Q....|
|00002f40| 00 11 f6 77 00 12 61 7c | 00 13 1d 53 00 13 b0 8e |...w..a||...S....|
|00002f50| 00 14 31 41 00 14 8b f2 | 00 15 41 15 00 15 bd 1f |..1A....|..A.....|
|00002f60| 00 16 a1 95 00 16 dd aa | 00 17 50 fb 00 17 7f 13 |........|..P.....|
|00002f70| 00 17 b2 4a 00 18 3e 49 | 00 18 ab 71 00 19 09 6f |...J..>I|...q...o|
|00002f80| 00 19 54 35 00 19 76 ed | 00 19 b1 60 00 1a 1e 72 |..T5..v.|...`...r|
|00002f90| 00 1a ae 4c 00 1a f4 74 | 00 1b 2f 0c 00 01 84 b8 |...L...t|../.....|
|00002fa0| 00 01 c4 6d 00 00 00 00 | 00 00 00 00 00 00 00 00 |...m....|........|
|00002fb0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002fc0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002fd0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002fe0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00002ff0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003000| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003010| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003020| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003030| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003040| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003050| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003060| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003070| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003080| 00 00 00 80 4c 49 53 54 | 00 00 0e 8a 00 00 00 02 |....LIST|........|
|00003090| 00 00 08 00 00 00 00 38 | 00 44 00 02 00 10 00 03 |.......8|.D......|
|000030a0| 20 00 0a 4b 00 00 00 38 | 00 00 00 00 00 00 00 00 | ..K...8|........|
|000030b0| 00 00 47 b8 00 1d 00 00 | 48 6d 00 1b 00 00 00 00 |..G.....|Hm......|
|000030c0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000030d0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000030e0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|000030f0| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 00 00 |........|........|
|00003100| 00 00 08 00 50 41 47 45 | 00 00 47 b8 00 00 0e 8a |....PAGE|..G.....|
|00003110| 56 c2 c2 f0 00 00 00 00 | 00 00 0b 76 e0 3f fd ff |V.......|...v.?..|
|00003120| 7f ff ff 7f fb ef fe ff | 5f fd ef ff bb f7 ff ff |........|_.......|
|00003130| ff eb ff fd fb df f7 f5 | ff ff fe f7 bb 7f fb fb |........|........|
|00003140| ff fb ff ff ff ff ff 37 | ff 7f df ff ff ff ff fd |.......7|........|
|00003150| ff ff ff fe df 77 fc ff | ff af fb ff 00 00 08 40 |.....w..|.......@|
|00003160| a0 7f ff 7f ff ff ff 7f | ff ff ff ff 7f ff ff ff |........|........|
|00003170| ff ff ff ff f7 ff ff ff | fb 9f ff ff ff ff ff f7 |........|........|
|00003180| ff ff ff ff ff ff ff ff | ff 7f ff bf ff ff ff ff |........|........|
|00003190| ff 7f ff ff ff ff ff ff | 7f ff ff fd ff ff ff ff |........|........|
|000031a0| 00 00 09 22 a0 17 fe 16 | fe cd cf 17 5e db 5c fb |..."....|....^.\.|
|000031b0| f7 f9 fb df ba fc 7f ab | d7 ef b7 fe f3 ff 73 8f |........|......s.|
|000031c0| ba 9f f7 e5 bf 7f f3 b6 | bd 7f 4e 28 f7 3f fd bf |........|..N(.?..|
|000031d0| 9a a1 9f 9b 3e 75 df 7f | e6 be f2 7f 51 7b be ed |....>u..|....Q{..|
|000031e0| ff ea fb ff 00 00 0f 65 | a0 5b 7a 8f 0d fa 49 26 |.......e|.[z...I&|
|000031f0| 05 ca d2 96 1f f7 6e 4d | a2 5e af d3 a2 ea 1d dc |......nM|.^......|
|00003200| ba 1f 1b 7b b6 de a7 76 | a6 fa c7 dd 35 b9 c0 3d |...{...v|....5..=|
|00003210| 9e 77 8b 31 b0 ef 1d bc | 0a 65 eb 37 76 ba 73 7f |.w.1....|.e.7v.s.|
|00003220| f0 71 38 df cb a7 c3 eb | 00 00 10 c1 a0 7b ff ef |.q8.....|.....{..|
|00003230| ff ff ff 7e ff ff ff ff | ff b7 ff ff 7a ff ff ff |...~....|....z...|
|00003240| d6 df ff ff ff ff 7f ff | ff ff f7 7f ff 3f f7 ff |........|.....?..|
|00003250| ff ff ff ff ff ff ff ff | ff f7 ff ff ff ff ff 7f |........|........|
|00003260| ff fe ff 7f fc ff fe ff | ff bf f7 ff 00 00 12 d7 |........|........|
|00003270| a0 3f bb 3f cf 77 f7 1f | f7 73 7c bf 1f 5d fb ff |.?.?.w..|.s|..]..|
|00003280| 7a 7e bf d9 56 df 9f ed | ef bf 77 3a fa bf 6d f7 |z~..V...|..w:..m.|
|00003290| 7d ff df 3f 37 5d f7 fa | ef f6 ba b9 1e f6 7a 1e |}..?7]..|......z.|
|000032a0| 7d 6f dc 7f fe 7b f3 3e | 5d 25 7f f6 ff bf c7 6f |}o...{.>|]%.....o|
|000032b0| 00 00 14 0d a0 7b ff 7e | ff ff f7 7f ff fb fe ff |.....{.~|........|
|000032c0| ff ff ff ff fb ff ff fb | f7 ff ff ff ff 9f 7f ff |........|........|
|000032d0| 7f ff ff ff bf 7f ff ff | 7f bf fc ff ff ff ff bf |........|........|
|000032e0| ff ff ff ff ff ff ff ff | ff ff f3 ff df f7 ff ff |........|........|
|000032f0| ff fe f7 ff 00 00 15 2b | a0 6b df 8f df db ef 7f |.......+|.k......|
|00003300| df fa ef ff 7f ff fe ff | fb 9f ff f9 bf f7 77 ff |........|......w.|
|00003310| ff df ff fb ff bf a7 ff | bf ff ff fb 7f 9f 7f ff |........|........|
|00003320| ff ff ff bf ff 67 ff fb | ff 7f 7f ff ff fb ff ff |.....g..|........|
|00003330| dd ff ff ff ef ef bf f7 | 00 00 16 09 a0 1e ed 4e |........|.......N|
|00003340| ff 6c ff ff 5d b3 4c ff | 13 fb ee 6b 3e 2e d9 b9 |.l..].L.|...k>...|
|00003350| fe a6 a6 fe f3 8c 7f ff | fa fd e5 74 96 b5 d3 b9 |........|...t....|
|00003360| 3f bb 14 7e f6 fa ff 7f | d7 c5 84 9f 3e df f7 77 |?..~....|....>..w|
|00003370| 67 7b d1 ef ec bf 57 ff | df da d3 e6 00 00 17 d0 |g{....W.|........|
|00003380| a0 7e fd ff ff ff ff ff | df ef 6e fb bf f7 ff ff |.~......|..n.....|
|00003390| ff fe ff fb b6 ef f7 ff | ff 7d f7 ff ff ff ff ff |........|.}......|
|000033a0| bf ff ff ff 7f ff 9f ff | ff ff ff af fb 7f ff ef |........|........|
|000033b0| ff f7 ff fb ff ff d1 ff | bd f7 7f ff cf ff ff ff |........|........|
|000033c0| 00 00 18 59 a0 7f ff ff | ff ff fb 7f ff ff ff ff |...Y....|........|
|000033d0| ff b7 ff ff ba ff ff ff | be ff 7f ff ff ff 7f ff |........|........|
|000033e0| ff ff ff 7f ff ff ff ff | ff ff ff ff ff ff ff ff |........|........|
|000033f0| ff ff ff ff 7f ff ff ff | ff ff ff 7f fd f7 ff ff |........|........|
|00003400| ff ff ff ff 00 00 19 75 | a0 67 ff af ff ff ef fe |.......u|.g......|
|00003410| bf fb fe bf 7f fd ef ff | bf ff ff fd ff ef b7 fe |........|........|
|00003420| ff ff 7f ff ff bf f7 fd | bd ff ff fd ff ff f7 7f |........|........|
|00003430| ff f9 ff ff fb 77 dd ff | ff 7f ff ff ff fb fb ff |.....w..|........|
|00003440| dd 7f ff ff ff ef ff ff | 00 00 1a ce a0 77 fd 7f |........|.....w..|
|00003450| ff f7 ff 77 fd fb fe ff | df ff ff fb ff 7f ff ff |...w....|........|
|00003460| ef ff ff ff fb ff ff f0 | ff ff f7 f7 b7 bf ff ff |........|........|
|00003470| 7f db fd ff f7 7f ef ff | df ff dd ff ff 7f ff ff |........|........|
|00003480| ff ff ff ff df f7 fe ff | ff af ff ff 00 00 1b 09 |........|........|
|00003490| a0 7d ec 92 09 7f 61 5b | f5 6a cf 9f 9b 2b ef 73 |.}....a[|.j...+.s|
|000034a0| a2 d6 d3 88 fe cb 54 5c | f3 1c 78 2a 3e bf ad c5 |......T\|..x*>...|
|000034b0| bf 35 57 7a 7d fc 6f fc | 35 b4 df 9a b9 f7 1d e8 |.5Wz}.o.|5.......|
|000034c0| ee 5d 17 77 f6 6a 95 77 | d4 33 15 df 5f 39 a3 f7 |.].w.j.w|.3.._9..|
|000034d0| 00 00 1c b6 a0 75 df 0e | cf 9d ab 7f 7f f2 ff ef |.....u..|........|
|000034e0| 7f ed fe ff fa fe df fb | f7 d9 b7 ff 7f 9f ff fa |........|........|
|000034f0| fe cf f5 ff bf 7f f3 ff | 7f 7d ff 79 ff ff fd bf |........|.}.y....|
|00003500| db 7f 4d de ff 77 7a f7 | 7f da f6 77 dd f7 ff fd |..M..wz.|...w....|
|00003510| ff fa 93 bb 00 00 1d 33 | a0 5e fd ff ff ff ef ff |.......3|.^......|
|00003520| fd ff 7e fb bf ff ef ff | bb fc ff fb f7 ef ff ff |..~.....|........|
|00003530| fb 7f 7f ff fe ff ff ff | ff ff ff ff 7f ff df ff |........|........|
|00003540| ff ff ff bf fb 7f ff ff | 7f f7 df fb f7 ff f1 ff |........|........|
|00003550| 9f f7 ff ff df ff ff ff | 00 00 1e 06 a0 2f ef ff |........|...../..|
|00003560| ff f7 ff 7f ff be fe bf | 7f fb ff ff 7b ff ff fd |........|....{...|
|00003570| e7 fd bf dd ff 7f fb f3 | ff bf f7 f5 f7 ff ff ff |........|........|
|00003580| 7f ff ff f3 ff f3 ff 73 | fb 7f fd ff 7f ff ff ff |.......s|........|
|00003590| ff 7f 7f ff ff f7 fe df | ff bf ff ff 00 00 1f 55 |........|.......U|
|000035a0| a0 6f ff 7f fe fb ff ff | df bb de fb 7f 6d fb ff |.o......|.....m..|
|000035b0| bf 7f ff fb c7 ef b7 fe | f7 ff fb f3 ff 7f 3f ff |........|......?.|
|000035c0| ff bf ff fa 77 bd f7 ff | ff ff ff de df 7b f6 ef |....w...|.....{..|
|000035d0| 7f ff df 7b ff fb f3 f7 | 7c f7 fe ff ff ef fe f6 |...{....||.......|
|000035e0| 00 00 20 80 a0 7f ff ff | ff ff ff 7f fb fb ff bf |.. .....|........|
|000035f0| 5f fd ff ff ff ff 7f fb | b7 ff bf fe fb df ff 7f |_.......|........|
|00003600| ff ff f7 fd bf ff fb fb | ff ff ff ff ff ff ff ff |........|........|
|00003610| df 7f bf ff 7f ff ff ff | ff 7f f3 7f d6 77 ff ff |........|.....w..|
|00003620| ff ff f3 bf 00 00 21 05 | a0 7f bf fe ef ee cb d7 |......!.|........|
|00003630| ff f3 fa fb ff ef ff ff | bb fe ff fb ff ff d7 fe |........|........|
|00003640| f3 df fb b7 fb fe f1 ff | bf 7f fb fb ff 7f bf fd |........|........|
|00003650| fd 7e ff 3f db 6f ff ff | 7f ff ff 7f ff f9 fa 7f |.~.?.o..|........|
|00003660| de ef 7f 7f ff fa fb ff | 00 00 22 6e a0 7f ff ff |........|.."n....|
|00003670| bf 7e ff 7f fb ef fe ff | 7f ff fe ff 7e 7e df fb |.~......|....~~..|
|00003680| ff b7 77 dd bb fc 7d fb | ff 7f f6 79 f7 f7 f7 ff |..w...}.|...y....|
|00003690| fd 7f ff ff bf fe ff 3f | df 7f f7 bf ff ff ff 7f |.......?|........|
|000036a0| ff df 73 ff dd b7 7e ff | ef ff f2 77 00 00 23 ed |..s...~.|...w..#.|
|000036b0| a0 7f ff 7f fd ee bf 5e | d1 eb de bf ff ff ff fb |.......^|........|
|000036c0| 7e fe ff f9 b7 ff ff 7f | fb ff ff ff ef af ff ff |~.......|........|
|000036d0| fe fd df fa ff fd fd ff | ff ff ff b3 fb ff fb ff |........|........|
|000036e0| ff fe fe 77 ff ff fe ff | d8 f7 3f ff ef ef f7 ab |...w....|..?.....|
|000036f0| 00 00 24 0a a0 5e 7f ff | a9 bb f7 5a 2f ff fb ff |..$..^..|...Z/...|
|00003700| d7 f7 7f 6b bf 77 7f d9 | ff de af e9 bb 5f fb 7b |...k.w..|....._.{|
|00003710| db af ed ff af fe c3 bf | 7b bb bf 7b fd ff bf fb |........|{..{....|
|00003720| df f7 ff bf ff af 7e 77 | 7f db 15 73 fc e3 55 ff |......~w|...s..U.|
|00003730| d6 27 d3 b7 00 00 25 7d | a0 7a c9 0f ff 29 af 5a |.'....%}|.z...).Z|
|00003740| 95 f6 7e eb 43 39 4f fb | 3b ec df f5 f7 c7 e7 ee |..~.C9O.|;.......|
|00003750| fb df f7 fb ba ed 37 71 | 86 37 d3 fb 3d fe 19 7e |......7q|.7..=..~|
|00003760| ef 3f ff f3 f3 eb f7 af | 1e b6 7e ff f7 e7 eb 77 |.?......|..~....w|
|00003770| 56 fd ff fd ff ae be a7 | 00 00 26 f2 a0 6e cd af |V.......|..&..n..|
|00003780| ff 63 af 1f dd 7a 6e fb | 5b bd 7f 7b 3b ee df ff |.c...zn.|[..{;...|
|00003790| d7 ff 35 ee bb 8e f7 f2 | ba 7f 27 73 a5 37 f3 fb |..5.....|..'s.7..|
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|00004770| 3e 3e 3e 3e 3e 3e 3e 3e | 3e 3e 3e 3e 3e 3e 3e 3e |>>>>>>>>|>>>>>>>>|
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|00004b10| 20 76 65 72 73 69 6f 6e | 0d 0d 20 20 20 3c 3c 3c | version|..   <<<|
|00004b20| 3c 3c 3c 3c 3c 3c 3c 3c | 3c 3c 3c 3c 3c 3c 3c 3c |<<<<<<<<|<<<<<<<<|
|00004b30| 3c 3c 3c 3c 3c 3c 3c 3c | 3c 3c 3c 2a 3e 3e 3e 3e |<<<<<<<<|<<<*>>>>|
|00004b40| 3e 3e 3e 3e 3e 3e 3e 3e | 3e 3e 3e 3e 3e 3e 3e 3e |>>>>>>>>|>>>>>>>>|
|00004b50| 3e 3e 3e 3e 3e 3e 3e 3e | 3e 3e 0d 0d 20 20 20 54 |>>>>>>>>|>>..   T|
|00004b60| 68 69 73 20 48 79 70 65 | 72 63 61 72 64 20 73 74 |his Hype|rcard st|
|00004b70| 61 63 6b 20 69 73 20 64 | 65 73 69 67 6e 65 64 20 |ack is d|esigned |
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|00004e10| 53 6f 20 69 74 20 67 6f | 65 73 2e 00 00 16 0d 20 |So it go|es..... |
|00004e20| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|00004e30| 20 20 20 20 20 20 20 48 | 6f 77 20 74 6f 20 75 73 |       H|ow to us|
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|00004e60| 3c 3c 3c 3c 3c 3c 3c 3c | 3c 3c 3c 3c 2a 3e 3e 3e |<<<<<<<<|<<<<*>>>|
|00004e70| 3e 3e 3e 3e 3e 3e 3e 3e | 3e 3e 3e 3e 3e 3e 3e 3e |>>>>>>>>|>>>>>>>>|
|00004e80| 3e 3e 3e 3e 3e 3e 3e 3e | 3e 3e 3e 0d 0d 20 20 20 |>>>>>>>>|>>>..   |
|00004e90| 4d 69 6e 69 4d 26 4d 20 | 73 68 6f 75 6c 64 20 62 |MiniM&M |should b|
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|00005130| 63 65 64 75 72 65 20 63 | 61 72 64 20 66 72 6f 6d |cedure c|ard from|
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|00005160| 65 2c 20 22 43 72 65 61 | 74 65 20 4d 26 4d 20 66 |e, "Crea|te M&M f|
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|00005180| 63 65 64 75 72 65 20 61 | 73 20 61 20 4d 61 63 57 |cedure a|s a MacW|
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|000051a0| 50 72 69 6e 74 20 41 4c | 4c 20 63 61 72 64 73 22 |Print AL|L cards"|
|000051b0| 20 69 73 20 74 68 65 20 | 73 61 6d 65 20 61 73 20 | is the |same as |
|000051c0| 22 50 72 69 6e 74 20 52 | 65 70 6f 72 74 22 2e 0d |"Print R|eport"..|
|000051d0| 00 00 3c 49 6e 66 6f 2c | 31 36 53 20 72 52 4e 41 |..<Info,|16S rRNA|
|000051e0| 20 73 65 71 27 69 6e 67 | 2c 41 64 76 61 6e 63 65 | seq'ing|,Advance|
|000051f0| 64 20 6d 69 6e 69 2d 70 | 72 65 70 2c 54 72 61 6e |d mini-p|rep,Tran|
|00005200| 73 66 6f 72 6d 61 74 69 | 6f 6e 2c 63 44 4e 41 20 |sformati|on,cDNA |
|00005210| 73 79 6e 74 68 65 73 69 | 73 2c 43 6f 6c 6f 6e 79 |synthesi|s,Colony|
|00005220| 20 6c 69 66 74 73 2c 43 | 73 43 6c 20 70 6c 61 73 | lifts,C|sCl plas|
|00005230| 6d 69 64 20 69 73 6f 6c | 23 32 2c 43 73 43 6c 20 |mid isol|#2,CsCl |
|00005240| 70 6c 61 73 6d 69 64 20 | 70 72 65 70 2c 43 73 43 |plasmid |prep,CsC|
|00005250| 6c 20 52 4e 41 20 69 73 | 6f 6c 2c 44 4d 53 20 74 |l RNA is|ol,DMS t|
|00005260| 72 65 61 74 69 6e 67 20 | 44 4e 41 2c 44 4e 61 73 |reating |DNA,DNas|
|00005270| 65 20 66 6f 6f 74 70 72 | 69 6e 74 69 6e 67 2c 64 |e footpr|inting,d|
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|00005290| 45 6e 64 2d 6c 61 62 65 | 6c 69 6e 67 20 44 4e 41 |End-labe|ling DNA|
|000052a0| 2c 45 6e 64 2d 6c 61 62 | 65 6c 69 6e 67 20 52 4e |,End-lab|eling RN|
|000052b0| 41 2c 45 74 42 72 20 73 | 70 6f 74 20 74 65 73 74 |A,EtBr s|pot test|
|000052c0| 2c 45 75 62 61 63 74 65 | 72 69 61 6c 20 52 4e 41 |,Eubacte|rial RNA|
|000052d0| 20 69 73 6f 6c 2c 45 78 | 6f 20 49 49 49 20 66 6f | isol,Ex|o III fo|
|000052e0| 6f 74 70 72 69 6e 74 69 | 6e 67 2c 46 69 67 75 72 |otprinti|ng,Figur|
|000052f0| 65 20 38 20 74 65 73 74 | 2c 46 69 6c 74 65 72 2d |e 8 test|,Filter-|
|00005300| 62 69 6e 64 69 6e 67 20 | 61 73 73 61 79 2c 47 65 |binding |assay,Ge|
|00005310| 6e 6f 6d 69 63 20 44 4e | 41 20 69 73 6f 6c 2c 47 |nomic DN|A isol,G|
|00005320| 69 61 6e 74 20 6d 69 6e | 69 2d 70 72 65 70 2c 47 |iant min|i-prep,G|
|00005330| 6c 79 6f 78 61 6c 20 67 | 65 6c 73 2c 4f 6c 64 20 |lyoxal g|els,Old |
|00005340| 68 79 62 72 69 64 69 7a | 61 74 69 6f 6e 2c 48 79 |hybridiz|ation,Hy|
|00005350| 62 72 69 64 69 73 61 74 | 69 6f 6e 2c 48 79 64 72 |bridisat|ion,Hydr|
|00005360| 6f 6c 79 7a 65 64 20 72 | 52 4e 41 2c 48 79 64 72 |olyzed r|RNA,Hydr|
|00005370| 6f 6c 79 7a 65 64 20 52 | 4e 41 2c 4d 31 33 20 63 |olyzed R|NA,M13 c|
|00005380| 6c 6f 6e 69 6e 67 2c 4d | 61 6e 69 61 74 69 73 21 |loning,M|aniatis!|
|00005390| 2c 4d 65 74 68 61 6e 6f | 67 65 6e 20 66 65 72 6d |,Methano|gen ferm|
|000053a0| 2c 4d 65 74 68 61 6e 6f | 67 65 6e 20 63 75 6c 74 |,Methano|gen cult|
|000053b0| 75 72 65 73 2c 4d 69 63 | 72 6f 2d 70 72 6f 74 65 |ures,Mic|ro-prote|
|000053c0| 69 6e 20 61 73 73 61 79 | 2c 4e 61 43 6c 20 70 70 |in assay|,NaCl pp|
|000053d0| 74 69 6f 6e 20 6f 66 20 | 52 4e 41 2c 4e 69 63 6b |tion of |RNA,Nick|
|000053e0| 20 74 72 61 6e 73 6c 61 | 74 69 6f 6e 2c 4e 6f 72 | transla|tion,Nor|
|000053f0| 74 68 65 72 6e 73 2c 42 | 61 74 63 68 20 6f 6c 69 |therns,B|atch oli|
|00005400| 67 6f 28 64 54 29 2c 4f | 6c 69 67 6f 28 64 54 29 |go(dT),O|ligo(dT)|
|00005410| 2d 63 65 6c 6c 75 6c 6f | 73 65 2c 52 4e 61 73 65 |-cellulo|se,RNase|
|00005420| 20 50 20 52 4e 41 20 70 | 72 65 70 2c 70 43 70 2d | P RNA p|rep,pCp-|
|00005430| 6c 61 62 65 6c 69 6e 67 | 20 52 4e 41 2c 50 6f 6c |labeling| RNA,Pol|
|00005440| 79 28 41 29 20 61 73 73 | 61 79 2c 50 6f 6c 79 28 |y(A) ass|ay,Poly(|
|00005450| 55 29 20 73 65 70 68 61 | 72 6f 73 65 2c 50 6f 6c |U) sepha|rose,Pol|
|00005460| 79 28 55 29 20 73 75 63 | 72 6f 73 65 20 67 72 61 |y(U) suc|rose gra|
|00005470| 64 73 2c 50 72 69 6d 65 | 72 20 65 78 74 65 6e 73 |ds,Prime|r extens|
|00005480| 69 6f 6e 2c 52 62 2b 2b | 20 63 6f 6d 70 65 74 65 |ion,Rb++| compete|
|00005490| 6e 74 20 63 65 6c 6c 73 | 2c 52 4e 41 20 73 75 63 |nt cells|,RNA suc|
|000054a0| 72 6f 73 65 20 67 72 61 | 64 73 2c 52 4e 41 50 20 |rose gra|ds,RNAP |
|000054b0| 61 73 73 61 79 2c 52 4e | 61 73 65 20 50 20 52 4e |assay,RN|ase P RN|
|000054c0| 41 20 61 73 73 61 79 2c | 53 65 71 75 65 6e 63 69 |A assay,|Sequenci|
|000054d0| 6e 67 20 67 65 6c 73 2c | 53 69 6e 67 6c 65 20 73 |ng gels,|Single s|
|000054e0| 74 65 70 20 52 4e 41 20 | 69 73 6f 6c 2c 53 6f 75 |tep RNA |isol,Sou|
|000054f0| 74 68 65 72 6e 73 2c 53 | 70 6f 74 20 62 6c 6f 74 |therns,S|pot blot|
|00005500| 73 2c 53 70 75 6e 20 63 | 6f 6c 75 6d 6e 73 2c 73 |s,Spun c|olumns,s|
|00005510| 73 44 4e 41 20 66 72 6f | 6d 20 70 54 5a 2f 70 42 |sDNA fro|m pTZ/pB|
|00005520| 53 2c 53 74 61 6e 64 61 | 72 64 20 6c 69 67 61 74 |S,Standa|rd ligat|
|00005530| 69 6f 6e 2c 54 37 20 74 | 72 61 6e 73 63 72 69 70 |ion,T7 t|ranscrip|
|00005540| 74 69 6f 6e 2c 54 41 42 | 20 72 65 73 74 2e 20 62 |tion,TAB| rest. b|
|00005550| 75 66 66 65 72 2c 55 56 | 2d 66 69 78 69 6e 67 20 |uffer,UV|-fixing |
|00005560| 66 69 6c 74 65 72 73 2c | 59 65 61 73 74 20 52 4e |filters,|Yeast RN|
|00005570| 41 20 69 73 6f 6c 00 49 | 6e 66 6f 00 00 00 00 00 |A isol.I|nfo.....|
|00005580| 00 00 23 80 43 41 52 44 | 00 00 08 40 00 00 00 00 |..#.CARD|...@....|
|00005590| 00 00 00 00 00 00 00 00 | 00 00 00 00 00 00 47 b8 |........|......G.|
|000055a0| 00 00 0a a6 00 00 00 00 | 00 00 00 00 00 06 00 00 |........|........|
|000055b0| 23 26 00 01 72 52 4e 41 | 20 73 65 71 75 65 6e 63 |#&..rRNA| sequenc|
|000055c0| 69 6e 67 20 75 73 69 6e | 67 20 72 65 76 65 72 73 |ing usin|g revers|
|000055d0| 65 20 74 72 61 6e 73 63 | 72 69 70 74 61 73 65 0d |e transc|riptase.|
|000055e0| 0d 00 00 1c 20 20 20 20 | 20 53 65 71 75 65 6e 63 |....    | Sequenc|
|000055f0| 69 6e 67 20 6f 66 20 72 | 52 4e 41 73 2c 20 70 72 |ing of r|RNAs, pr|
|00005600| 69 6d 61 72 69 6c 79 20 | 31 36 53 20 72 52 4e 41 |imarily |16S rRNA|
|00005610| 20 61 74 20 74 68 69 73 | 20 74 69 6d 65 2c 20 69 | at this| time, i|
|00005620| 73 20 61 20 75 73 65 66 | 75 6c 20 6d 65 74 68 6f |s a usef|ul metho|
|00005630| 64 20 66 6f 72 20 71 75 | 69 63 6b 6c 79 20 64 65 |d for qu|ickly de|
|00005640| 74 65 72 6d 69 6e 69 6e | 67 20 74 68 65 20 70 68 |terminin|g the ph|
|00005650| 6c 79 6f 67 65 6e 65 74 | 69 63 20 70 6c 61 63 65 |lyogenet|ic place|
|00005660| 6d 65 6e 74 20 6f 66 20 | 61 6e 20 6f 72 67 61 6e |ment of |an organ|
|00005670| 69 73 6d 2e 20 20 42 65 | 63 61 75 73 65 20 6f 66 |ism.  Be|cause of|
|00005680| 20 74 68 65 20 70 72 65 | 73 65 6e 63 65 20 6f 66 | the pre|sence of|
|00005690| 20 68 69 67 68 6c 79 20 | 63 6f 6e 73 65 72 76 65 | highly |conserve|
|000056a0| 64 20 72 65 67 69 6f 6e | 73 20 69 6e 20 74 68 65 |d region|s in the|
|000056b0| 20 31 36 53 20 72 52 4e | 41 2c 20 75 6e 69 76 65 | 16S rRN|A, unive|
|000056c0| 72 73 61 6c 20 6f 6c 69 | 67 6f 6e 75 63 6c 65 6f |rsal oli|gonucleo|
|000056d0| 74 69 64 65 20 70 72 69 | 6d 65 72 73 20 61 72 65 |tide pri|mers are|
|000056e0| 20 61 76 61 69 6c 61 62 | 6c 65 20 66 6f 72 20 73 | availab|le for s|
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|00005740| 73 69 74 75 61 74 65 64 | 20 69 6e 20 64 6f 77 6e |situated| in down|
|00005750| 73 74 72 65 61 6d 20 6f | 66 20 72 65 67 69 6f 6e |stream o|f region|
|00005760| 73 20 74 68 61 74 20 61 | 72 65 20 71 75 69 74 65 |s that a|re quite|
|00005770| 20 76 61 72 69 61 62 6c | 65 3b 20 74 68 65 73 65 | variabl|e; these|
|00005780| 20 61 72 65 20 73 65 71 | 75 65 6e 63 65 73 20 77 | are seq|uences w|
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|000057a0| 73 65 66 75 6c 20 66 6f | 72 20 70 68 79 6c 6f 67 |seful fo|r phylog|
|000057b0| 65 6e 65 74 69 63 20 61 | 6e 61 6c 79 73 69 73 2e |enetic a|nalysis.|
|000057c0| 20 20 54 68 65 20 6d 65 | 74 68 6f 64 20 64 65 73 |  The me|thod des|
|000057d0| 63 72 69 62 65 64 20 68 | 65 72 65 20 69 73 2c 20 |cribed h|ere is, |
|000057e0| 6f 66 20 63 6f 75 72 73 | 65 2c 20 61 6c 73 6f 20 |of cours|e, also |
|000057f0| 61 70 6c 69 63 61 62 6c | 65 20 66 6f 72 20 32 33 |aplicabl|e for 23|
|00005800| 53 20 72 52 4e 41 20 73 | 65 71 75 65 6e 63 69 6e |S rRNA s|equencin|
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|00005820| 70 72 69 61 74 65 20 6f | 6c 69 67 6f 6e 75 63 6c |priate o|ligonucl|
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|00005840| 72 65 20 75 73 65 64 2e | 20 20 54 68 65 20 6d 65 |re used.|  The me|
|00005850| 74 68 6f 64 20 63 61 6e | 20 65 76 65 6e 20 62 65 |thod can| even be|
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|00005870| 63 69 6e 67 20 61 62 75 | 6e 64 61 6e 74 20 6d 52 |cing abu|ndant mR|
|00005880| 4e 41 73 20 2d 20 69 6e | 20 74 68 69 73 20 63 61 |NAs - in| this ca|
|00005890| 73 65 2c 20 69 74 20 6d | 69 67 68 74 20 62 65 20 |se, it m|ight be |
|000058a0| 75 73 65 66 75 6c 20 74 | 6f 20 6d 6f 64 69 66 79 |useful t|o modify|
|000058b0| 20 74 68 65 20 6d 65 74 | 68 6f 64 20 62 79 20 75 | the met|hod by u|
|000058c0| 73 69 6e 67 20 65 6e 64 | 2d 6c 61 62 65 6c 65 64 |sing end|-labeled|
|000058d0| 20 70 72 69 6d 65 72 73 | 20 72 61 74 68 65 72 20 | primers| rather |
|000058e0| 74 68 61 6e 20 69 6e 63 | 6f 72 70 6f 72 61 74 69 |than inc|orporati|
|000058f0| 6e 67 20 72 61 64 69 6f | 6c 61 62 65 6c 65 64 20 |ng radio|labeled |
|00005900| 64 4e 54 50 73 20 69 6e | 74 6f 20 74 68 65 20 72 |dNTPs in|to the r|
|00005910| 65 61 63 74 69 6f 6e 20 | 6d 69 78 65 73 2e 0d 20 |eaction |mixes.. |
|00005920| 20 20 20 20 54 68 65 20 | 6d 6f 73 74 20 69 6d 70 |    The |most imp|
|00005930| 6f 72 74 61 6e 74 20 70 | 61 72 74 20 6f 66 20 74 |ortant p|art of t|
|00005940| 68 69 73 20 70 72 6f 63 | 65 64 75 72 65 20 69 73 |his proc|edure is|
|00005950| 20 74 68 65 20 52 4e 41 | 20 69 73 6f 6c 61 74 69 | the RNA| isolati|
|00005960| 6f 6e 2e 20 20 55 73 65 | 20 52 4e 61 73 65 2d 66 |on.  Use| RNase-f|
|00005970| 72 65 65 20 74 65 63 68 | 6e 69 71 75 65 21 20 20 |ree tech|nique!  |
|00005980| 4f 74 68 65 72 77 69 73 | 65 2c 20 74 68 65 20 70 |Otherwis|e, the p|
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|000059a0| 65 73 73 65 6e 74 69 61 | 6c 6c 79 20 64 69 66 66 |essentia|lly diff|
|000059b0| 65 72 65 6e 74 20 66 72 | 6f 6d 20 63 68 61 69 6e |erent fr|om chain|
|000059c0| 20 74 65 72 6d 69 6e 61 | 74 69 6f 6e 20 73 65 71 | termina|tion seq|
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|000059e0| 20 74 65 6d 70 6c 61 74 | 65 73 2e 0d 20 20 20 20 | templat|es..    |
|000059f0| 20 49 66 20 74 68 65 20 | 73 65 71 75 65 6e 63 65 | If the |sequence|
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|00005a10| 74 6f 20 69 6e 74 65 72 | 70 72 65 74 65 2c 20 74 |to inter|prete, t|
|00005a20| 72 79 20 72 65 2d 70 72 | 65 63 69 70 69 74 61 74 |ry re-pr|ecipitat|
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|00005a40| 4d 20 4e 61 43 6c 20 28 | 30 43 20 6f 76 65 72 6e |M NaCl (|0C overn|
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|00005a60| 6c 6f 77 20 4d 57 20 52 | 4e 41 73 2e 20 20 41 6c |low MW R|NAs.  Al|
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|00005ad0| 67 2f 6d 6c 20 73 74 6f | 63 6b 20 63 6f 6e 63 65 |g/ml sto|ck conce|
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|00005af0| 35 58 20 68 79 62 72 69 | 64 69 7a 61 74 69 6f 6e |5X hybri|dization|
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|00005b20| 20 20 20 31 30 30 6d 4d | 20 4b 43 6c 20 20 20 20 |   100mM| KCl    |
|00005b30| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
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|00005b90| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
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|00005c20| 54 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |T       |        |
|00005c30| 20 20 20 20 20 20 20 31 | 30 6d 4d 20 45 44 54 41 |       1|0mM EDTA|
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|00005c70| 20 63 79 61 6e 6f 6c 0d | 20 20 20 20 20 20 20 20 | cyanol.|        |
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|00005d10| 6f 6c 0d 0d 4f 6c 69 67 | 6f 6e 75 63 6c 65 6f 74 |ol..Olig|onucleot|
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|00005d50| 20 20 70 72 69 6d 65 72 | 20 35 31 39 20 20 20 20 |  primer| 519    |
|00005d60| 20 35 27 2d 47 57 41 41 | 54 41 43 43 47 43 47 47 | 5'-GWAA|TACCGCGG|
|00005d70| 43 4b 47 43 54 47 2d 33 | 27 0d 20 20 20 20 20 70 |CKGCTG-3|'.     p|
|00005d80| 72 69 6d 65 72 20 39 31 | 35 20 20 20 20 20 35 27 |rimer 91|5     5'|
|00005d90| 2d 47 43 43 43 43 43 47 | 59 43 41 41 54 54 43 43 |-GCCCCCG|YCAATTCC|
|00005da0| 54 2d 33 27 0d 20 20 20 | 20 20 70 72 69 6d 65 72 |T-3'.   |  primer|
|00005db0| 20 31 33 39 31 20 20 20 | 35 27 2d 47 41 43 47 47 | 1391   |5'-GACGG|
|00005dc0| 47 43 47 47 54 47 54 47 | 54 52 43 41 2d 33 27 0d |GCGGTGTG|TRCA-3'.|
|00005dd0| 0d 50 52 45 50 41 52 41 | 54 49 4f 4e 20 4f 46 20 |.PREPARA|TION OF |
|00005de0| 4e 55 43 4c 45 4f 54 49 | 44 45 20 4d 49 58 45 53 |NUCLEOTI|DE MIXES|
|00005df0| 0d 0d 31 2e 20 20 46 6f | 72 20 64 4e 54 50 73 2c |..1.  Fo|r dNTPs,|
|00005e00| 20 64 69 73 73 6f 6c 76 | 65 20 31 30 6d 67 20 69 | dissolv|e 10mg i|
|00005e10| 6e 20 31 6d 6c 20 31 30 | 6d 4d 20 54 72 69 73 2c |n 1ml 10|mM Tris,|
|00005e20| 20 70 48 38 2e 33 2e 20 | 20 20 46 6f 72 20 64 64 | pH8.3. |  For dd|
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|00005e40| 75 6d 6f 6c 65 73 20 69 | 6e 20 33 30 30 75 6c 20 |umoles i|n 300ul |
|00005e50| 31 30 6d 4d 20 54 72 69 | 73 2c 20 70 48 38 2e 33 |10mM Tri|s, pH8.3|
|00005e60| 0d 0d 32 2e 20 44 69 6c | 75 74 65 20 34 75 6c 20 |..2. Dil|ute 4ul |
|00005e70| 6f 66 20 74 68 69 73 20 | 69 6e 74 6f 20 32 6d 6c |of this |into 2ml|
|00005e80| 20 31 30 6d 4d 20 54 72 | 69 73 2c 20 70 48 20 37 | 10mM Tr|is, pH 7|
|00005e90| 2e 34 2c 20 61 6e 64 20 | 72 65 61 64 20 74 68 65 |.4, and |read the|
|00005ea0| 20 61 62 73 6f 72 62 61 | 6e 63 65 20 61 74 20 74 | absorba|nce at t|
|00005eb0| 68 61 74 20 28 64 29 64 | 4e 54 50 73 20 61 62 73 |hat (d)d|NTPs abs|
|00005ec0| 6f 72 70 74 69 6f 6e 20 | 6d 61 78 69 6d 75 6d 20 |orption |maximum |
|00005ed0| 2d 20 74 68 65 20 28 64 | 29 64 4e 54 50 20 63 6f |- the (d|)dNTP co|
|00005ee0| 6e 63 65 6e 74 72 61 74 | 69 6f 6e 20 63 61 6e 20 |ncentrat|ion can |
|00005ef0| 62 65 20 63 61 6c 63 75 | 6c 61 74 65 64 20 61 73 |be calcu|lated as|
|00005f00| 3a 0d 0d 20 20 20 20 28 | 61 62 73 6f 72 62 61 6e |:..    (|absorban|
|00005f10| 63 65 29 28 64 69 6c 75 | 74 69 6f 6e 20 66 61 63 |ce)(dilu|tion fac|
|00005f20| 74 6f 72 29 0d 20 20 20 | 20 2d 2d 2d 2d 2d 2d 2d |tor).   | -------|
|00005f30| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|00005f40| 2d 2d 2d 2d 2d 2d 20 20 | 20 3d 20 20 63 6f 6e 63 |------  | =  conc|
|00005f50| 65 6e 74 72 61 74 69 6f | 6e 20 69 6e 20 6d 4d 0d |entratio|n in mM.|
|00005f60| 20 20 20 20 20 20 6d 4d | 20 65 78 74 69 6e 74 69 |      mM| extinti|
|00005f70| 6f 6e 20 63 6f 65 66 66 | 69 63 69 65 6e 74 0d 0d |on coeff|icient..|
|00005f80| 54 68 65 20 63 6f 6e 73 | 74 61 6e 74 73 20 6e 65 |The cons|tants ne|
|00005f90| 65 64 65 64 20 61 72 65 | 3a 0d 0d 6e 75 63 6c 65 |eded are|:..nucle|
|00005fa0| 6f 74 69 64 65 20 20 20 | 20 20 20 61 62 73 6f 72 |otide   |   absor|
|00005fb0| 70 74 69 6f 6e 20 6d 61 | 78 20 28 6e 6d 29 20 20 |ption ma|x (nm)  |
|00005fc0| 20 20 6d 4d 20 65 78 74 | 69 6e 63 74 69 6f 6e 20 |  mM ext|inction |
|00005fd0| 63 6f 65 66 66 0d 20 20 | 20 64 41 54 50 20 20 20 |coeff.  | dATP   |
|00005fe0| 20 20 20 20 20 20 20 20 | 20 20 20 20 32 35 39 20 |        |    259 |
|00005ff0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|00006000| 20 20 20 20 31 35 2e 32 | 0d 20 20 20 64 43 54 50 |    15.2|.   dCTP|
|00006010| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 32 |        |       2|
|00006020| 37 32 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |72      |        |
|00006030| 20 20 20 20 20 20 20 20 | 39 2e 31 0d 20 20 20 64 |        |9.1.   d|
|00006040| 47 54 50 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |GTP     |        |
|00006050| 20 20 32 35 33 20 20 20 | 20 20 20 20 20 20 20 20 |  253   |        |
|00006060| 20 20 20 20 20 20 20 20 | 20 20 31 33 2e 37 0d 20 |        |  13.7. |
|00006070| 20 20 64 54 54 50 20 20 | 20 20 20 20 20 20 20 20 |  dTTP  |        |
|00006080| 20 20 20 20 20 32 36 37 | 20 20 20 20 20 20 20 20 |     267|        |
|00006090| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 39 2e |        |      9.|
|000060a0| 36 0d 20 20 20 64 49 54 | 50 20 20 20 20 20 20 20 |6.   dIT|P       |
|000060b0| 20 20 20 20 20 20 20 20 | 32 34 39 20 20 20 20 20 |        |249     |
|000060c0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|000060d0| 31 32 2e 32 0d 20 20 5b | 53 5d 64 41 54 50 20 20 |12.2.  [|S]dATP  |
|000060e0| 20 20 20 20 20 20 20 20 | 20 20 20 32 35 39 20 20 |        |   259  |
|000060f0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|00006100| 20 20 20 31 34 2e 38 0d | 20 20 20 64 64 41 54 50 |   14.8.|   ddATP|
|00006110| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 32 35 |        |      25|
|00006120| 39 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |9       |        |
|00006130| 20 20 20 20 20 20 31 35 | 2e 32 0d 20 20 20 64 64 |      15|.2.   dd|
|00006140| 43 54 50 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |CTP     |        |
|00006150| 20 32 37 32 20 20 20 20 | 20 20 20 20 20 20 20 20 | 272    |        |
|00006160| 20 20 20 20 20 20 20 20 | 20 20 39 2e 31 0d 20 20 |        |  9.1.  |
|00006170| 20 64 64 47 54 50 20 20 | 20 20 20 20 20 20 20 20 | ddGTP  |        |
|00006180| 20 20 20 20 32 35 33 20 | 20 20 20 20 20 20 20 20 |    253 |        |
|00006190| 20 20 20 20 20 20 20 20 | 20 20 20 20 31 33 2e 37 |        |    13.7|
|000061a0| 0d 20 20 20 64 64 54 54 | 50 20 20 20 20 20 20 20 |.   ddTT|P       |
|000061b0| 20 20 20 20 20 20 20 32 | 36 37 20 20 20 20 20 20 |       2|67      |
|000061c0| 20 20 20 20 20 20 20 20 | 20 20 20 20 20 20 20 20 |        |        |
|000061d0| 39 2e 36 0d 20 0d 33 2e | 20 44 69 6c 75 74 65 20 |9.6. .3.| Dilute |
|000061e0| 74 68 65 20 69 6e 69 74 | 69 61 6c 20 73 6f 6c 75 |the init|ial solu|
|000061f0| 74 69 6f 6e 20 74 6f 20 | 31 30 6d 4d 20 62 79 20 |tion to |10mM by |
|00006200| 61 64 64 69 6e 67 20 31 | 30 6d 4d 20 54 72 69 73 |adding 1|0mM Tris|
|00006210| 2c 20 70 48 38 2e 33 2e | 0d 0d 34 2e 20 54 6f 20 |, pH8.3.|..4. To |
|00006220| 74 68 65 20 61 6c 70 68 | 61 2d 74 68 69 6f 2d 64 |the alph|a-thio-d|
|00006230| 41 54 50 20 73 6f 6c 75 | 74 69 6f 6e 2c 20 61 64 |ATP solu|tion, ad|
|00006240| 64 20 31 2f 31 30 30 30 | 20 76 6f 6c 75 6d 65 20 |d 1/1000| volume |
|00006250| 28 31 75 6c 2f 6d 6c 29 | 20 31 4d 20 44 54 54 2e |(1ul/ml)| 1M DTT.|
|00006260| 0d 0d 35 2e 20 53 74 6f | 72 65 20 61 74 20 2d 37 |..5. Sto|re at -7|
|00006270| 30 43 2e 0d 0d 36 2e 20 | 20 4d 61 6b 65 20 73 6d |0C...6. | Make sm|
|00006280| 61 6c 6c 20 28 7e 35 30 | 30 75 6c 29 20 77 6f 72 |all (~50|0ul) wor|
|00006290| 6b 69 6e 67 20 73 74 6f | 63 6b 73 20 6f 66 20 65 |king sto|cks of e|
|000062a0| 61 63 68 20 6f 66 20 74 | 68 65 20 66 6f 6c 6c 6f |ach of t|he follo|
|000062b0| 77 69 6e 67 20 69 6e 20 | 31 30 6d 4d 20 54 72 69 |wing in |10mM Tri|
|000062c0| 73 2c 20 70 48 38 2e 33 | 3a 20 31 6d 4d 20 64 64 |s, pH8.3|: 1mM dd|
|000062d0| 43 54 50 2c 20 31 6d 4d | 20 64 64 47 54 50 2c 20 |CTP, 1mM| ddGTP, |
|000062e0| 31 6d 4d 20 64 64 54 54 | 50 2c 20 61 6e 64 20 30 |1mM ddTT|P, and 0|
|000062f0| 2e 31 6d 4d 20 64 64 41 | 54 50 2e 0d 0d 37 2e 20 |.1mM ddA|TP...7. |
|00006300| 20 53 69 78 20 6e 75 63 | 6c 65 6f 74 69 64 65 20 | Six nuc|leotide |
|00006310| 6d 69 78 65 73 20 61 72 | 65 20 6e 65 65 64 65 64 |mixes ar|e needed|
|00006320| 20 66 6f 72 20 52 54 20 | 73 65 71 75 65 6e 63 69 | for RT |sequenci|
|00006330| 6e 67 20 28 6e 75 6d 62 | 65 72 73 20 61 72 65 20 |ng (numb|ers are |
|00006340| 69 6e 20 75 6c 29 3a 0d | 0d 20 20 20 20 20 20 20 |in ul):.|.       |
|00006350| 20 20 20 20 20 20 20 20 | 47 2d 6d 69 78 20 20 43 |        |G-mix  C|
|00006360| 2d 6d 69 78 20 20 54 2d | 6d 69 78 20 20 41 2d 6d |-mix  T-|mix  A-m|
|00006370| 69 78 20 20 6e 6f 2d 64 | 64 20 6d 69 78 20 20 63 |ix  no-d|d mix  c|
|00006380| 68 61 73 65 0d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |hase.---|--------|
|00006390| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000063a0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000063b0| 2d 2d 2d 2d 2d 2d 2d 2d | 2d 2d 2d 2d 2d 2d 2d 2d |--------|--------|
|000063c0| 2d 0d 31 30 6d 4d 20 64 | 47 54 50 20 20 20 20 20 |-.10mM d|GTP     |
|000063d0| 20 31 30 75 6c 20 20 20 | 20 31 30 20 20 20 20 20 | 10ul   | 10     |
|000063e0| 31 30 20 20 20 20 20 31 | 30 20 20 20 20 20 20 20 |10     1|0       |
|000063f0| 31 30 20 20 20 20 20 20 | 20 31 34 30 0d 31 30 6d |10      | 140.10m|
+--------+-------------------------+-------------------------+--------+--------+